Amebiasis: Biology and Pathogenesis of Entamoeba by Tomoyoshi Nozaki, Alok Bhattacharya PDF

By Tomoyoshi Nozaki, Alok Bhattacharya

ISBN-10: 4431551999

ISBN-13: 9784431551997

ISBN-10: 4431552006

ISBN-13: 9784431552000

This e-book records and provides new advancements within the examine of amebiasis, one of many missed tropical illnesses. approximately 50 million humans world wide are contaminated with the pathogen Entamoeba histolytica, inflicting large-scale morbidity and mortality rather in constructing nations. This booklet may also help clinicians for larger prognosis and administration of the ailment, researchers for beginning study tasks on many of the poorly understood elements of the ailment and the pathogen, and scholars for updating their wisdom. the topics coated variety from genomics and molecular and phone biology to drug resistance and new drug improvement, highlighting significant advances lately in our realizing because of swift growth in genomic and different biomedical applied sciences, similar to visualization of molecular tactics. many of the chapters supply contemporary info in response to most recent courses. a number of chapters describe many of the severe methodological matters that might be invaluable for college students and researchers attracted to entering into the sector. The contributing authors comprise just about all the lively researchers and clinicians from world wide. This booklet should be an invaluable basic fabric and a invaluable resource of knowledge for an individual drawn to realizing amebiasis, its prognosis, and remedy. it is going to even be helpful to those that have an interest in studying in regards to the biology of early branching eukaryotes and protist pathogens.

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Extra resources for Amebiasis: Biology and Pathogenesis of Entamoeba

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J Eukaryot Microbiol 40:340–344 28. Silberman JD, Clark CG, Diamond LS, Sogin ML (1999) Phylogeny of the genera Entamoeba and Endolimax as deduced from small-subunit ribosomal RNA sequences. Mol Biol Evol 16:1740–1751 29. Clark CG, Kaffashian F, Tawari B, Windsor JJ, Twigg-Flesner A, Davies-Morel MCG, Blessmann J, Ebert F, Pesche B, Le Van A, Jackson CJ, Macfarlane L, Tannich E (2006) New insights into the phylogeny of Entamoeba species provided by analysis of four new smallsubunit rRNA genes. Int J Syst Evol Microbiol 56:2235–2239 30.

The genome is larger than that of E. histolytica or E. dispar (approximately 41 million base pairs of DNA in 1,149 scaffolds) and is slightly less AT rich (approximately 70 % AT); 11,549 annotated genes span approximately 38 % of the genome. 6 Interspecific Variation Among Entamoeba Genomes Given the amount of sequence data used in the E. histolytica genome assembly, it is likely that the majority of genes of HM-1:IMSS genes are represented in the assembly. The gene content of the organism reflects its biology and has been reviewed in 3 The Genomics of Entamoebae: Insights and Challenges 37 detail [30].

HAPPY mapping has been applied to E. histolytica, but the results were difficult to interpret, largely because of the extreme repetitiveness of the E. histolytica genome (Dr. Elisabet Caler, personal communication). Optical mapping is a technique to produce ordered restriction maps of single DNA molecules [42]. Optical mapping has been applied to E. histolytica, with some success (Dr. Elisabet Caler, personal communication). A small number of putative linkage groups (23) could be determined. However, it remains difficult to map sequence scaffolds, particularly the many short scaffolds in the E.

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Amebiasis: Biology and Pathogenesis of Entamoeba by Tomoyoshi Nozaki, Alok Bhattacharya

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